Human PLAU/uPA (Urokinase-Type Plasminogen Activator) ELISA Kit
The Human PLAU (Urokinase-Type Plasminogen Activator) ELISA Kit is specifically designed for the precise measurement of PLAU levels in human samples, including serum, plasma, and cell culture supernatants. With its superior sensitivity and specificity, this ELISA kit ensures accurate and consistent results, making it an indispensable tool for various research purposes.PLAU, also known as urokinase-type plasminogen activator, is a key enzyme involved in the regulation of fibrinolysis and cell migration. Its dysregulation has been implicated in various pathological conditions such as cancer, inflammation, and thrombosis. By detecting PLAU levels, researchers can gain valuable insights into the mechanisms underlying these diseases and potentially identify novel therapeutic targets.Overall, the Human PLAU ELISA Kit offers a reliable and efficient way to quantify PLAU levels in human samples, facilitating research efforts in understanding the role of this enzyme in health and disease.
Product Name:
Human PLAU/uPA (Urokinase-Type Plasminogen Activator) ELISA Kit
SKU:
HUES02753
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
37.5 pg/mL
Detection range:
62.5-4000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
