The Human SIGA (Secretory Immunoglobulin A) CLIA Kit is a highly reliable and sensitive assay designed for the accurate detection of secretory immunoglobulin A levels in human biological samples. This CLIA kit is suitable for serum, plasma, and cell culture supernatants, providing researchers with precise and reproducible results for a variety of research applications.Secretory immunoglobulin A is an important component of the body's immune system, playing a key role in mucosal immunity and defense against pathogens. Abnormal levels of SIGA have been associated with various diseases and conditions, making it a valuable biomarker for studying immune responses and developing targeted therapies.With its unparalleled sensitivity and specificity, the Human SIGA CLIA Kit from AssayGenie is the perfect tool for researchers looking to assess SIGA levels in human samples with confidence and accuracy. This kit is easy to use and provides rapid results, making it an essential asset for immunology research and clinical diagnostics.
Product Name:
Human sIgA (Secretory Immunoglobulin A) CLIA Kit
SKU:
HUES00731
Size:
96 Assays
Detection Method:
Chemiluminescence
Assay type:
Sandwich-CLIA
Assay time:
3 h 30 min
Sensitivity:
37.5 pg/mL
Detection range:
62.5-4000 pg/mL
Reovery:
80%-120%
This kit uses a sandwich chemiluminescence immunoassay (CLIA) principle. The microplate is pre-coated with an antibody specific to the target protein. Standards or samples are added to the wells and bind to the immobilized antibody. A biotinylated detection antibody is then added, followed by HRP-conjugated streptavidin to form a sandwich complex. After washing to remove unbound components, a chemiluminescent substrate is added. The HRP enzyme catalyzes a light-emitting reaction. The intensity of the emitted light is directly proportional to the concentration of the target protein in the sample. The signal is measured using a luminometer, and the concentration of the analyte is calculated based on a standard curve.
