Human SRD5a2 (Steroid 5 Alpha Reductase 2) ELISA Kit
The Human SRD5A2 (Steroid 5 alpha-reductase 2) ELISA Kit is a reliable tool for the precise measurement of SRD5A2 levels in human serum, plasma, and cell culture supernatants. This kit offers excellent sensitivity and specificity, providing consistent and accurate results for a variety of research applications.SRD5A2 is an enzyme crucial for the conversion of testosterone to dihydrotestosterone (DHT), a potent androgen involved in various physiological processes. Dysregulation of SRD5A2 activity has been linked to conditions such as prostate cancer, male pattern baldness, and benign prostatic hyperplasia, highlighting its importance as a biomarker for studying and developing treatments for these conditions.By using the Human SRD5A2 ELISA Kit, researchers can gain valuable insights into the role of SRD5A2 in health and disease, paving the way for innovative therapeutic strategies and personalized medicine approaches.
Product Name:
Human SRD5a2 (Steroid 5 Alpha Reductase 2) ELISA Kit
SKU:
HUES03378
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
