Human Trypsin Pan/PRSS1-2-3 ELISA Kit (SBRS1199)

Human Trypsin Pan/PRSS1-2-3 PharmaGenie ELISA Kit (SBRS1199)

SKU:

SBRS1199

Product Type:

ELISA Kit

Reactivity:

Human

Applications:

ELISA

ELISA Type:

Sandwich

Description

Description

Datasheet

Human Trypsin Pan/PRSS1-2-3 PharmaGenie ELISA - Kit Data

Product SKU:	SBRS1199
Size:	96T
Application:	This ELISA antibody pair recognizes Human Trypsin Pan.
Uniprot:	P07477 / P07478 / P35030
Gene ID:	5644 / 5645 / 5646
Gene Names:	PRSS1 \| PRSS2 \| PRSS3 \| PRSS4 \| TRP1 \| TRY1 \| TRY2 \| TRY3 \| TRY4 \| TRYP1<>\|TRYP2
Synonyms:	Trypsin-1 (EC 3.4.21.4) (Beta-trypsin) (Cationic trypsinogen) (Serine protease 1) (Trypsin I) [Cleaved into: Alpha-trypsin chain 1 Alpha-trypsin chain 2] / Trypsin-2 (EC 3.4.21.4) (Anionic trypsinogen) (Serine protease 2) (Trypsin II) / Trypsin-3 (EC 3.4.21.4) (Brain trypsinogen) (Mesotrypsinogen) (Serine protease 3) (Serine protease 4) (Trypsin III) (Trypsin IV)
Target Species:	Human
Compatible Sample Types:	Cell Culture Supernatants, Plasma, Serum
Design Principle:	Sandwich-based
Method of Detection:	Colorimetric
Quantitative/Semi-Quantitative:	Quantitative
Range:	30.72 pg/ml - 7500 pg/ml
Sensitivity:	30 pg/ml
Recommended Dilution:	200 fold
Storage/Stability:	The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Kit Components:

1.	Pre-Coated 96-well Strip Microplate
2.	Wash Buffer
3.	Stop Solution
4.	Assay Diluent(s)
5.	Lyophilized Standard
6.	Biotinylated Detection Antibody
7.	Streptavidin-Conjugated HRP
8.	TMB One-Step Substrate

Other materials and equipment required:

The Assay Genie Human Trypsin Pan/PRSS1-2-3 ELISA Kit will require other equipment and materials to carry out the assay. Please see list below for further details.

Distilled or deionized water
Precision pipettes to deliver 2 ul to 1 ul volumes
Adjustable 1-25 ul pipettes for reagent preparation
100 ul and 1 liter graduated cylinders
Tubes to prepare standard and sample dilutions
Absorbent paper
Microplate reader capable of measuring absorbance at 450nm
Log-log graph paper or computer and software for ELISA data analysis

Kit Protocol:

1.	Prepare all reagents, samples and standards as instructed in the manual.
2.	Add 100 ul of standard or sample to each well.
3.	Incubate 2.5 h at RT or O/N at 4°C.
4.	Add 100 ul of prepared biotin antibody to each well.
5.	Incubate 1 h at RT.
6.	Add 100 ul of prepared Streptavidin solution to each well.
7.	Incubate 45 min at RT.
8.	Add 100 ul of TMB One-Step Substrate Reagent to each well.
9.	Incubate 30 min at RT.
10.	Add 50 ul of Stop Solution to each well.
11.	Read at 450 nm immediately.