The Human VEGFR3 (FLT4) ELISA Kit from Assay Genie is a powerful tool for the precise measurement of Vascular Endothelial Growth Factor Receptor 3 levels in human samples. This kit boasts exceptional sensitivity and specificity, guaranteeing accurate and consistent results for various research purposes.VEGFR3, also known as FLT4, is a key player in vascular endothelial cell growth, playing a critical role in angiogenesis and lymphangiogenesis. Dysregulation of VEGFR3 has been implicated in numerous diseases, including cancer and vascular disorders, highlighting its significance as a potential therapeutic target and diagnostic marker.Trust in the Human VEGFR3 (FLT4) ELISA Kit from Assay Genie for reliable and insightful data on Vascular Endothelial Growth Factor Receptor 3 levels, advancing your research in the fields of vascular biology, cancer biology, and beyond.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.