The Mouse VEGFR3 (FLT4) CLIA Kit is specifically designed for the quantitative measurement of vascular endothelial growth factor receptor 3 (VEGFR3) levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.VEGFR3 is a vital receptor involved in mediating vascular endothelial cell growth factor signaling, playing a crucial role in angiogenesis and lymphangiogenesis processes. Dysregulation of VEGFR3 has been implicated in various diseases such as cancer, lymphedema, and inflammatory disorders, highlighting its importance as a biomarker for studying these conditions and developing potential therapeutic interventions.Overall, the Mouse VEGFR3 (FLT4) CLIA Kit provides a reliable tool for researchers to investigate VEGFR3 levels and its role in angiogenesis-related pathways, offering valuable insights into disease mechanisms and potential therapeutic targets.
This kit uses a sandwich chemiluminescence immunoassay (CLIA) principle. The microplate is pre-coated with an antibody specific to the target protein. Standards or samples are added to the wells and bind to the immobilized antibody. A biotinylated detection antibody is then added, followed by HRP-conjugated streptavidin to form a sandwich complex. After washing to remove unbound components, a chemiluminescent substrate is added. The HRP enzyme catalyzes a light-emitting reaction. The intensity of the emitted light is directly proportional to the concentration of the target protein in the sample. The signal is measured using a luminometer, and the concentration of the analyte is calculated based on a standard curve.
