Human VIP (Vasoactive Intestinal Peptide) ELISA Kit
The Human VIP (Vasoactive Intestinal Peptide) ELISA Kit is specifically designed for the precise measurement of VIP levels in human samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results, making it an excellent choice for various research applications.VIP is a vital neuropeptide that plays a critical role in regulating numerous physiological functions, including smooth muscle relaxation, immune response modulation, and hormone secretion. Dysregulation of VIP has been linked to various disorders such as inflammation, autoimmune diseases, and neurological disorders, highlighting its significance as a biomarker for studying and developing treatments for these conditions.In conclusion, the Human VIP ELISA Kit is a valuable tool for researchers looking to study the role of VIP in human health and disease, offering reliable and precise measurements for insightful and impactful research findings.
Product Name:
Human VIP (Vasoactive Intestinal Peptide) ELISA Kit
SKU:
HUES03058
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Competitive
Assay type:
Competitive-ELISA
Assay time:
2 h 30 min
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with the target antigen. Standards or samples are added along with a biotinylated detection antibody. The target antigen present in the sample competes with the immobilized antigen for binding to the detection antibody. After incubation, Avidin-Horseradish Peroxidase (HRP) conjugate is added. Free components are washed away. The substrate solution is then added, resulting in a color change. The intensity of the color is inversely proportional to the concentration of the target antigen in the sample. The reaction is stopped by the addition of stop solution, and the color changes from blue to yellow. The optical density (OD) is measured at 450 nm ± 2 nm. The concentration of the target protein is calculated by comparing the OD values of the samples to the standard curve.
