Anti-Insulin Antibody (CAB19066)

SKU:
CAB19066
€129 - €349

Description

Technical Manual

Anti-Insulin Antibody

Product Name:Anti-Insulin Antibody
Product SKU:CAB19066
Size:20uL, 50 uL
Host Species:Rabbit
Purification Method:Affinity purification
Isotype:IgG
Background:

After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010]

Immunogen:A synthesized peptide derived from human Insulin
Sequence:

Email for sequence

Gene ID:3630
Swiss Prot:P01308
Synonyms:IDDM; IDDM1; IDDM2; ILPR; IRDN; MODY10; INS; Insulin; insulin
Calculated MW:kDa
Observed MW:12kDa
Reactivity:Mouse, Rat
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application Key:Western blotting Immunohistochemistry Immunofluorescence 
Positive Samples:Mouse pancreas, Rat pancreas
Cellular Location:

Product Images

Anti-Insulin Antibody (CAB19066)

Western blot - Insulin Rabbit mAb (CAB19066)

Uniprot Information

UniProt Protein Function:Insulin: Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. Heterodimer of a B chain and an A chain linked by two disulfide bonds. Belongs to the insulin family.
UniProt Protein Details:

Protein type:Secreted; Secreted, signal peptide; Hormone

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: extracellular space; Golgi lumen; endoplasmic reticulum lumen; extracellular region

Molecular Function:identical protein binding; insulin-like growth factor receptor binding; protein binding; protease binding; hormone activity; insulin receptor binding

Biological Process: positive regulation of nitric oxide biosynthetic process; positive regulation of vasodilation; glucose homeostasis; activation of NF-kappaB transcription factor; positive regulation of glucose import; negative regulation of protein oligomerization; regulation of protein localization; cell-cell signaling; negative regulation of gluconeogenesis; acute-phase response; regulation of transmembrane transporter activity; negative regulation of NAD(P)H oxidase activity; positive regulation of mitosis; activation of protein kinase B; positive regulation of nitric-oxide synthase activity; negative regulation of acute inflammatory response; glucose metabolic process; positive regulation of lipid biosynthetic process; positive regulation of protein amino acid autophosphorylation; positive regulation of cell growth; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of cell differentiation; negative regulation of protein catabolic process; regulation of amino acid metabolic process; negative regulation of glycogen catabolic process; wound healing; positive regulation of glycogen biosynthetic process; positive regulation of cellular protein metabolic process; glucose transport; regulation of protein secretion; negative regulation of lipid catabolic process; regulation of transcription, DNA-dependent; positive regulation of MAPKKK cascade; negative regulation of vasodilation; positive regulation of cell proliferation; negative regulation of proteolysis; negative regulation of protein secretion; MAPKKK cascade; negative regulation of fatty acid metabolic process; alpha-beta T cell activation; endocrine pancreas development; G-protein coupled receptor protein signaling pathway; positive regulation of protein kinase B signaling cascade; cellular protein metabolic process; fatty acid homeostasis; positive regulation of glycolysis; insulin receptor signaling pathway; energy reserve metabolic process; positive regulation of insulin receptor signaling pathway; regulation of insulin secretion; positive regulation of DNA replication; positive regulation of cytokine secretion; positive regulation of cell migration

Disease: Diabetes Mellitus, Insulin-dependent, 2; Diabetes Mellitus, Permanent Neonatal; Hyperproinsulinemia; Maturity-onset Diabetes Of The Young, Type 10

NCBI Summary:After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010]
UniProt Code:P01308
NCBI GenInfo Identifier:124617
NCBI Gene ID:3630
NCBI Accession:P01308.1
UniProt Secondary Accession:P01308,Q5EEX2,
UniProt Related Accession:P01308
Molecular Weight:110
NCBI Full Name:Insulin
NCBI Synonym Full Names:insulin
NCBI Official Symbol:INS 
NCBI Official Synonym Symbols:IDDM; ILPR; IRDN; IDDM1; IDDM2; MODY10 
NCBI Protein Information:insulin; proinsulin; preproinsulin
UniProt Protein Name:Insulin
Protein Family:Insulin
UniProt Gene Name:INS 
UniProt Entry Name:INS_HUMAN
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Additional Information

Product type:
Antibody
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Metabolism
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