The KCNN3/SK3 Monoclonal Antibody (CAB23419) is a high-quality antibody developed for reliable detection and analysis of target proteins. Action potentials in vertebrate neurons are followed by an afterhyperpolarization (AHP) that may persist for several seconds and may have profound consequences for the firing pattern of the neuron. Each component of the AHP is kinetically distinct and is mediated by different calcium-activated potassium channels. This gene belongs to the KCNN family of potassium channels. It encodes an integral membrane protein that forms a voltage-independent calcium-activated channel, which is thought to regulate neuronal excitability by contributing to the slow component of synaptic AHP. This gene contains two CAG repeat regions in the coding sequence. It was thought that expansion of one or both of these repeats could lead to an increased susceptibility to schizophrenia or bipolar disorder, but studies indicate that this is probably not the case. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Rat samples.
Product Name:
KCNN3/SK3 Monoclonal Antibody
SKU:
CAB23419
Size:
100μL, 20μL
Reactivity:
Rat
Clone Number:
ARC60792
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:1000 - 1:4000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
SK3, ZLS3, hSK3, SKCA3, KCa2.3, KCNN3/SK3
Positive Sample:
Rat brain
Cellular Localization:
Membrane, Multi-Pass Membrane Protein.
Calculated MW:
81 kDa
Observed MW:
81 kDa
Action potentials in vertebrate neurons are followed by an afterhyperpolarization (AHP) that may persist for several seconds and may have profound consequences for the firing pattern of the neuron. Each component of the AHP is kinetically distinct and is mediated by different calcium-activated potassium channels. This gene belongs to the KCNN family of potassium channels. It encodes an integral membrane protein that forms a voltage-independent calcium-activated channel, which is thought to regulate neuronal excitability by contributing to the slow component of synaptic AHP. This gene contains two CAG repeat regions in the coding sequence. It was thought that expansion of one or both of these repeats could lead to an increased susceptibility to schizophrenia or bipolar disorder, but studies indicate that this is probably not the case. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
3782
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Rat brain using KCNN3/SK3 Rabbit mAb (CAB23419) at 1:2000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30 s.
![APC Anti-Human CD4 Antibody [SK3] (AGEL3444)](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/590x590/products/221565/607347/apc-anti-human-cd4-antibody-sk3__33278.1707500926.jpg?c=2 "APC Anti-Human CD4 Antibody [SK3] (AGEL3444)")
![PE/Cyanine7 Anti-Human CD4 Antibody [SK3] (AGEL3446)](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/590x590/products/221567/606929/pecyanine7-anti-human-cd4-antibody-sk3__60008.1707499611.jpg?c=2 "PE/Cyanine7 Anti-Human CD4 Antibody [SK3] (AGEL3446)")