Description
KIT Antibody (CAB7521)
The KIT Antibody (CAB7521) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, offers high reactivity with human samples and has been validated for use in Western blot applications. By binding to the CD300A protein, researchers can easily detect and analyze its expression in various cell types, making it an ideal choice for studies in immunology and cancer research.CD300A, also known as an immune inhibitory receptor, plays a vital role in immune homeostasis by regulating inflammation and inhibiting allergic reactions.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
| Product Name: | KIT Antibody |
| SKU: | CAB7521 |
| Size: | 20μL, 100μL |
| Reactivity: | Human, Mouse, Rat |
| Conjugate: | Unconjugated |
| Immunogen: | Recombinant protein (or fragment).This information is considered to be commercially sensitive. | ||||
| Sequence: | SSPY PGMP VDSK FYKM IKEG FRML SPEH APAE MYDI MKTC WDAD PLKR PTFK QIVQ LIEK QISE STNH IYSN LANC SPNR QKPV VDHS VRIN SVGS TASS SQPL LVHD DV | ||||
| Tested Applications: | WB ELISA | ||||
| Recommended Dilution: |
| ||||
| Synonyms: | PBT, SCFR, C-Kit, CD117, MASTC, CD117/c-Kit |
| Positive Sample: | Mouse lung, Mouse brain |
| Cellular Localization: | Cell Membrane, Cytoplasm, Single-Pass Type I Membrane Protein. |
| Calculated MW: | 110kDa |
| Observed MW: | 120kDa/145kDa |
This gene encodes a receptor tyrosine kinase. This gene was initially identified as a homolog of the feline sarcoma viral oncogene v-kit and is often referred to as proto-oncogene c-Kit. The canonical form of this glycosylated transmembrane protein has an N-terminal extracellular region with five immunoglobulin-like domains, a transmembrane region, and an intracellular tyrosine kinase domain at the C-terminus. Upon activation by its cytokine ligand, stem cell factor (SCF), this protein phosphorylates multiple intracellular proteins that play a role in in the proliferation, differentiation, migration and apoptosis of many cell types and thereby plays an important role in hematopoiesis, stem cell maintenance, gametogenesis, melanogenesis, and in mast cell development, migration and function. This protein can be a membrane-bound or soluble protein. Mutations in this gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism. Multiple transcript variants encoding different isoforms have been found for this gene.
| Purification Method | Affinity purification |
| Gene ID | 3815 |
| RRID | AB_2768049 |
| Buffer Information | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3. |
 | Western blot analysis of various lysates, using CD117/c-Kit Rabbit pAb (CAB7521) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 20s. |
