The [KO Validated] Acetyl-HMGB1-K29 Antibody (CAB16002) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits and validated for use in various applications including Western blot and immunohistochemistry, specifically targets the acetylated form of HMGB1 at lysine 29.HMGB1 is a highly conserved nuclear protein implicated in a variety of cellular processes, including DNA repair, transcriptional regulation, and inflammation. Acetylation of HMGB1 at specific residues, such as lysine 29, has been shown to modulate its functions and interactions with other proteins, leading to diverse cellular responses.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
[KO Validated] Acetyl-HMGB1-K29 Antibody
SKU:
CAB16002
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBIF/ICCELISA
Recommended Dilution:
WB
1:500 - 1:2000
IF/ICC
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
This gene encodes a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein.
Purification Method
Affinity purification
Gene ID
3146
RRID
AB_2768214
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Immunofluorescence analysis of C6 cells using Acetyl-HMGB1-K29 Rabbit pAb (CAB16002) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using Acetyl-HMGB1-K29 Rabbit pAb (CAB16002) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
