Anti-Acetyl-HMGB1-K29 Antibody (CAB16002)

Antibody Name:	Anti-Acetyl-HMGB1-K29 Antibody [KO Validated]
Antibody SKU:	CAB16002
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic acetylated peptide around K29 of human HMGB1.

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T

Immunogen:	A synthetic acetylated peptide around K29 of human HMGB1.
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	3146
Uniprot:	P09429
Cellular Location:	Cell membrane, Chromosome, Cytoplasm, Endoplasmic reticulum-Golgi intermediate compartment, Endosome, Extracellular side, Nucleus, Peripheral membrane protein, Secreted
Calculated MW:	24kDa
Observed MW:	28kDa

	Western blot analysis of extracts from normal (control) and Acetyl-HMGB1-K29 knockout (KO) 293T cells, using Acetyl-HMGB1-K29 antibody (CAB16002) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 5min.
	Immunofluorescence analysis of C6 cells using [KO Validated] Acetyl-HMGB1-K29 Rabbit pAb (CAB16002) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of NIH-3T3 cells using [KO Validated] Acetyl-HMGB1-K29 Rabbit pAb (CAB16002) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of U-2 OS cells using [KO Validated] Acetyl-HMGB1-K29 Rabbit pAb (CAB16002) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Synonyms:

HMG-1, HMG1, HMG3, SBP-1, HMGB1

Background:

This gene encodes a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein.

UniProt Protein Function:	Multifunctional redox sensitive protein with various roles in different cellular compartments. In the nucleus is one of the major chromatin-associated non-histone proteins and acts as a DNA chaperone involved in replication, transcription, chromatin remodeling, V(D)J recombination, DNA repair and genome stability. Proposed to be an universal biosensor for nucleic acids. Promotes host inflammatory response to sterile and infectious signals and is involved in the coordination and integration of innate and adaptive immune responses. In the cytoplasm functions as sensor and/or chaperone for immunogenic nucleic acids implicating the activation of TLR9-mediated immune responses, and mediates autophagy. Acts as danger associated molecular pattern (DAMP) molecule that amplifies immune responses during tissue injury (PubMed:27362237). Released to the extracellular environment can bind DNA, nucleosomes, IL-1 beta, CXCL12, AGER isoform 2/sRAGE, lipopolysaccharide (LPS) and lipoteichoic acid (LTA), and activates cells through engagement of multiple surface receptors. In the extracellular compartment fully reduced HMGB1 (released by necrosis) acts as a chemokine, disulfide HMGB1 (actively secreted) as a cytokine, and sulfonyl HMGB1 (released from apoptotic cells) promotes immunological tolerance (PubMed:23519706, PubMed:23446148, PubMed:23994764, PubMed:25048472). Has proangiogdenic activity. May be involved in platelet activation. Binds to phosphatidylserine and phosphatidylethanolamide. Bound to RAGE mediates signaling for neuronal outgrowth. May play a role in accumulation of expanded polyglutamine (polyQ) proteins such as huntingtin (HTT) or TBP (PubMed:23303669, PubMed:25549101).
NCBI Summary:	This gene encodes a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2015]
UniProt Code:	P09429
NCBI GenInfo Identifier:	123369
NCBI Gene ID:	3146
NCBI Accession:	P09429.3
UniProt Secondary Accession:	P09429,Q14321, Q5T7C3, Q6IBE1, A5D8W9,
UniProt Related Accession:	P09429
Molecular Weight:	24,894 Da
NCBI Full Name:	High mobility group protein B1
NCBI Synonym Full Names:	high mobility group box 1
NCBI Official Symbol:	HMGB1
NCBI Official Synonym Symbols:	HMG1; HMG3; HMG-1; SBP-1
NCBI Protein Information:	high mobility group protein B1
UniProt Protein Name:	High mobility group protein B1
UniProt Synonym Protein Names:	High mobility group protein 1; HMG-1
Protein Family:	High mobility group protein
UniProt Gene Name:	HMGB1

Antibodies	ELISA Kits
Anti-HMGB1 Antibody (CAB0719)[KO Validated]	Human HMGB1 ELISA Kit
Anti-HMGB1 Antibody (CAB2553)[KO Validated]	Human HMGB-1 (High mobility group protein B1) CLIA Kit (HUES00881)
Anti-Acetyl-HMGB1-K28 Antibody (CAB16001)	Human High mobility group protein B1 (HMGB1) ELISA Kit
Anti-Acetyl-HMGB1-K30 Antibody (CAB16003)
Anti-Acetyl-HMGB1-K90 Antibody (CAB16004)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)