Mouse ACE (Angiotensin I Converting Enzyme) ELISA Kit
The Mouse ACE (Angiotensin I Converting Enzyme) ELISA Kit is a cutting-edge assay designed for the precise quantitative detection of ACE levels in various mouse biological samples. ACE plays a crucial role in the renin-angiotensin system, influencing blood pressure regulation, electrolyte balance, and cardiovascular health. Accurate measurement of ACE is essential for deepening our understanding of its involvement in cardiovascular physiology and pathophysiology related to hypertension, heart failure, and renal diseases. By employing Assay Genie's Mouse ACE ELISA Kit, researchers can expect outstanding sensitivity and specificity, ensuring accurate and reproducible results. This kit is manufactured under stringent quality control standards to deliver reliable and robust performance, making it an invaluable tool for investigating the intricate mechanisms of ACE in cardiovascular health and exploring potential therapeutic strategies. Its user-friendly design enhances usability, making it an excellent choice for cardiovascular research studies.
Product Name:
Mouse ACE (Angiotensin I Converting Enzyme) ELISA Kit
SKU:
AEES00208
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
