Description
Mouse Angiogenesis Array (24 targets) (SARB0072)
The Mouse Angiogenesis Array (SARB0072) is a comprehensive tool for studying angiogenesis in mouse models. This array kit allows for the simultaneous detection of 24 key angiogenesis-related targets in a single experiment, providing valuable insights into the molecular mechanisms underlying this complex process.With targets including growth factors, receptors, and cytokines involved in angiogenesis, this array offers a high-throughput and cost-effective solution for researchers studying vascular development, tumor growth, and wound healing in mice.
The array is easy to use, requiring only a small amount of sample for analysis, and provides reliable and reproducible results.Whether investigating the effects of new drugs on angiogenesis or exploring the role of specific signaling pathways in vascular development, the Mouse Angiogenesis Array offers a powerful tool for advancing research in this critical area of biology. By enabling the simultaneous analysis of multiple targets, this array saves time and resources while providing a comprehensive view of angiogenic processes in mouse models.
| Product Sku: | SARB0072 |
| Size: | 2, 4, or 8 |
| Species Detected: | Mouse |
| Number of Targets Detected: | 24 |
| Gene Symbols: | CCL11, CCL2, CSF1, CSF2, CSF3, CXCL9, FASLG, FGF2, IFNG, IGF2|IL12A, IL13, IL1A, IL1B, IL6, IL9, LEP, PF4, THPO, TIMP1, TIMP2, TNF, TPO |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
Eotaxin-1 (CCL11)
| Fas Ligand (TNFSF6)
| bFGF
| GCSF
| GM-CSF
|
IFN-gamma
| IGF-2
| IL-1 alpha (IL-1 F1)
| IL-1 beta (IL-1 F2)
| IL-12 p40/p70
|
IL-12 p70
| IL-13
| IL-6
| IL-9
| Leptin
|
MCP-1 (CCL2)
| M-CSF
| MIG (CXCL9)
| Platelet Factor 4 (CXCL4)
| TIMP-1
|
TIMP-2
| TNF alpha
| Thrombopoietin (TPO)
| VEGF-A
| |
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Mouse Angiogenesis Antibody Array C1 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Biotinylated Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated Streptavidin |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
