The Mouse BK (Bradykinin) ELISA Kit (MOES00781) offered by AssayGenie is a cutting-edge research tool designed for the precise measurement of bradykinin levels in mouse serum, plasma, and tissue culture supernatants. This advanced kit boasts exceptional sensitivity and specificity, ensuring accurate and reproducible results that are vital for a wide array of experimental applications.Bradykinin is a key signaling molecule in inflammation and pain pathways, playing a crucial role in vasodilation, smooth muscle contraction, and permeability of blood vessels. Dysregulation of bradykinin levels has been implicated in various pathological conditions, including hypertension, edema, and inflammatory disorders. As such, this ELISA kit is an invaluable resource for researchers studying the role of bradykinin in health and disease, paving the way for potential therapeutic interventions and advancements in the field of biomedical research.Experience unparalleled precision and reliability with the Mouse BK (Bradykinin) ELISA Kit (MOES00781) from AssayGenie - your trusted partner in cutting-edge research solutions.
Product Name:
Mouse BK (Bradykinin) ELISA Kit
SKU:
MOES00781
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Competitive
Assay type:
Competitive-ELISA
Assay time:
2 h 30 min
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with the target antigen. Standards or samples are added along with a biotinylated detection antibody. The target antigen present in the sample competes with the immobilized antigen for binding to the detection antibody. After incubation, Avidin-Horseradish Peroxidase (HRP) conjugate is added. Free components are washed away. The substrate solution is then added, resulting in a color change. The intensity of the color is inversely proportional to the concentration of the target antigen in the sample. The reaction is stopped by the addition of stop solution, and the color changes from blue to yellow. The optical density (OD) is measured at 450 nm ± 2 nm. The concentration of the target protein is calculated by comparing the OD values of the samples to the standard curve.
