The Mouse Casp3 (Caspase-3) ELISA Kit is specially designed for the accurate measurement of caspase-3 levels in mouse samples, including serum, plasma, and cell culture supernatants. This kit is highly sensitive and specific, ensuring reliable and reproducible results for a wide range of research applications.Caspase-3 is a key enzyme involved in the process of apoptosis, playing a crucial role in programmed cell death. Dysregulation of caspase-3 activity has been linked to various diseases, including cancer, neurodegenerative disorders, and autoimmune conditions. Therefore, the measurement of caspase-3 levels is essential for understanding the underlying mechanisms of these diseases and developing targeted therapies.The Mouse Casp3 ELISA Kit from Assay Genie provides researchers with a powerful tool for studying apoptosis and investigating the role of caspase-3 in disease progression. With its high sensitivity and specificity, this kit offers accurate and dependable results, making it a valuable addition to any laboratory conducting research in the field of cell biology and molecular medicine.
Product Name:
Mouse CASP3 (Caspase 3) ELISA Kit
SKU:
MOES00811
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.1 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
