Mouse FABP4 (Fatty Acid Binding Protein 4, Adipocyte) ELISA Kit
The Mouse FABP4 (Fatty Acid Binding Protein 4) Adipocyte ELISA Kit is specifically designed for the quantitative detection of FABP4 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reliable results for various research applications.FABP4 is a key protein involved in lipid metabolism and adipocyte function, playing a critical role in regulating glucose and lipid homeostasis. Dysregulation of FABP4 has been linked to obesity, diabetes, and cardiovascular diseases, making it a valuable biomarker for studying metabolic disorders and developing potential therapeutic interventions.With its user-friendly features and high-performance capabilities, the Mouse FABP4 Adipocyte ELISA Kit is an essential tool for researchers studying adipocyte biology, metabolic disorders, and potential therapeutic targets in mouse models.
Product Name:
Mouse FABP4 (Fatty Acid Binding Protein 4, Adipocyte) ELISA Kit
SKU:
MOES01685
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.47 ng/mL
Detection range:
0.78-50 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
