The Mouse FN (Fibronectin) ELISA Kit is specifically designed for the precise measurement of fibronectin levels in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and consistent results, making it an invaluable tool for various research purposes.Fibronectin is a key component of the extracellular matrix and plays a crucial role in cell adhesion, migration, and tissue remodeling. It is involved in various physiological and pathological processes, including wound healing, embryonic development, and cancer progression. By accurately measuring fibronectin levels, researchers can better understand its role in these processes and potentially identify new therapeutic targets.Overall, the Mouse FN (Fibronectin) ELISA Kit provides researchers with a reliable and efficient method to study the role of fibronectin in health and disease, ultimately advancing our knowledge and potentially leading to new treatments.
Product Name:
Mouse FN (Fibronectin) ELISA Kit
SKU:
MOES01029
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
