The Mouse GHR ELISA Kit is expertly designed for the precise quantitative assessment of Growth Hormone Receptor (GHR) levels in a variety of biological samples. GHR, the receptor for growth hormone, plays a crucial role in mediating the actions of growth hormone in various cellular and physiological processes essential for growth, metabolism, and development. Accurate quantification of GHR is imperative for understanding its function in growth regulation, metabolic control, and cellular signaling pathways. By measuring GHR levels, researchers can elucidate the molecular mechanisms underlying growth hormone activity and its impacts on cellular proliferation, differentiation, and hormone response. With exceptional sensitivity and specificity, the Mouse GHR ELISA Kit offered by Assay Genie ensures precise and reproducible results. Manufactured under strict quality control standards, this kit delivers reliable performance and user-friendly operation, making it an ideal solution for investigative studies aimed at unraveling the intricate functions of the Growth Hormone Receptor in growth and metabolic processes.
Product Name:
Mouse GHR (Growth Hormone Receptor) ELISA Kit
SKU:
AEES00253
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
