The Mouse GHR (Growth Hormone Receptor) ELISA Kit - High Sensitivity is an advanced research tool tailored for the precise quantitative analysis of Growth Hormone Receptor (GHR) levels present in various biological samples obtained from mouse specimens. As a fundamental component of the growth hormone signaling pathway, GHR plays a pivotal role in cellular growth, metabolism regulation, and development processes, making it a key target in studies exploring growth regulation and related biological functions in mice. This high-sensitivity ELISA kit offers researchers a cutting-edge platform to investigate the intricate mechanisms by which GHR modulates growth hormone-related signaling pathways in mouse models. By accurately measuring GHR levels, researchers can gain valuable insights into the molecular interactions and cellular responses mediated by GHR, shedding light on its implications in growth regulation, metabolic functions, and disease processes in mouse experimental systems. The Mouse GHR ELISA Kit - High Sensitivity by Assay Genie ensures exceptional sensitivity and specificity, enabling researchers to generate reliable and reproducible data. With robust performance and user-friendly protocols, this kit is an optimal choice for researchers seeking to unravel the complexities of GHR-mediated signaling pathways and their implications for growth-related processes in mouse biology.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
