Description
Mouse Growth Factor Array (20 targets) (SARB0087)
The Mouse Growth Factor Array (SARB0087) is a comprehensive research tool that allows for the simultaneous detection and quantification of 20 different growth factors in mouse samples. This array provides researchers with a valuable tool for studying the complex interactions between these growth factors and their roles in various biological processes.With targets including insulin-like growth factors, epidermal growth factor, and transforming growth factor-beta, this array covers a wide range of growth factors involved in cell growth, proliferation, and differentiation. Whether studying developmental processes, cancer progression, or tissue regeneration, this array offers a convenient and efficient way to analyze multiple growth factors in a single experiment.
Designed for use with mouse samples, this growth factor array provides high sensitivity and specificity, allowing for accurate and reliable results. It is a valuable resource for researchers in fields such as developmental biology, oncology, and regenerative medicine, providing insights into the complex signaling networks that govern cellular behavior. Overall, the Mouse Growth Factor Array (SARB0087) is a versatile and powerful tool for advancing our understanding of growth factor signaling and its impact on various physiological and pathological processes in mouse models.
| Product Sku: | SARB0087 |
| Size: | 2, 4, or 8 |
| Species Detected: | Mouse |
| Number of Targets Detected: | 20 |
| Gene Symbols: | AREG, CSF1, CSF2, CSF3, EGF, FGF2, FIGF, FLT1, FLT4, HGF|IGF1, IGF2, IGFBP2, IGFBP3, IGFBP5, IGFBP6, IL2, IL7, KITLG, VEGFA |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | IGF Signaling / p53 Signaling |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
Amphiregulin
| bFGF
| EGF
| GCSF
| GM-CSF
|
HGF
| IGF-1
| IGF-2
| IGFBP-2
| IGFBP-3
|
IGFBP-5
| IGFBP-6
| IL-2
| IL-7
| M-CSF
|
SCF
| VEGF-A
| VEGF-D
| VEGFR1
| VEGFR3
|
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Mouse Growth Factor Antibody Array C2 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Biotinylated Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated Streptavidin |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
