The Mouse IFN-gamma (Interferon-gamma) CLIA Kit is a highly reliable and sensitive assay designed for the accurate quantification of IFN-gamma levels in mouse serum, plasma, and cell culture supernatants. This kit ensures precise and reproducible results, making it ideal for a wide range of research applications.IFN-gamma is a key cytokine involved in immune responses, playing a critical role in combating infections and regulating inflammation. Dysregulation of IFN-gamma levels has been linked to various autoimmune diseases, infectious diseases, and cancer, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.
Overall, the Mouse IFN-gamma CLIA Kit provides researchers with a powerful tool for studying the immune response and investigating the role of IFN-gamma in various disease states. Its high sensitivity and specificity make it a valuable asset for advancing our understanding of immune function and disease pathology in mouse models.
Product Name:
Mouse IFN-gamma (Interferon Gamma) CLIA Kit
SKU:
MOES00045
Target:
Mouse IFN-gamma (Interferon Gamma)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse IFN-γ. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IFN-γ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IFN-γ, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse IFN-γ. You can calculate the concentration of Mouse IFN-γ in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-111
91-106
99-113
Average (%)
104
97
105
1:4
Range (%)
86-101
99-114
100-115
Average (%)
93
106
105
1:8
Range (%)
101-117
84-98
102-114
Average (%)
109
91
108
1:16
Range (%)
90-104
83-97
90-105
Average (%)
95
90
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-104
97
EDTA plasma (n=5)
86-101
93
Cell culture media (n=5)
89-102
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
25.81
53.18
224.04
25.32
55.07
208.56
Standard deviation
2.22
5.42
14.9
2.12
4.87
14.79
C V (%)
8.6
10.19
6.65
8.37
8.84
7.09
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse IFN-γ concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse IFN-γ in samples. No significant cross-reactivity or interference between Mouse IFN-γ and analogues was observed.
