The Mouse Immunoglobulin G1 (IgG1) ELISA Kit is meticulously crafted for the precise quantitative measurement of IgG1 levels in a variety of biological samples. Immunoglobulin G1 is a prominent antibody subclass known for its crucial role in mediating immune responses, including pathogen recognition, opsonization, and antigen neutralization. This ELISA kit provides exceptional sensitivity and specificity, facilitating accurate determination of IgG1 levels and furthering the understanding of its immunological functions.
Manufactured under rigorous quality control standards, the kit ensures robust and reproducible results, making it an ideal tool for research endeavors aiming to explore the intricate mechanisms of immune responses governed by IgG1 antibodies.
Product Name:
Mouse IgG1 (Immunoglobulin G1) ELISA Kit
SKU:
AEES00230
Target:
Mouse IgG1 (Immunoglobulin G1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
3.75 ng/mL
Detection range:
6.25-400 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IgG1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IgG1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IgG1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IgG1. You can calculate the concentration of Mouse IgG1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-102
97-109
98-111
Average (%)
98
103
105
1:4
Range (%)
91-107
98-111
92-106
Average (%)
99
104
98
1:8
Range (%)
86-99
86-97
100-111
Average (%)
92
93
103
1:16
Range (%)
82-93
99-108
84-100
Average (%)
90
105
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-108
101
EDTA plasma (n=5)
85-99
93
Cell culture media (n=5)
99-110
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
16.75
46.08
163.26
18.94
44.05
194.01
Standard deviation
0.95
2.16
8.39
1.13
2.04
10.32
C V (%)
5.67
4.69
5.14
5.97
4.63
5.32
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse IgG1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse IgG1 in samples. No significant cross-reactivity or interference between Mouse IgG1 and analogues was observed.
