The Mouse IL-6 CLIA Kit is a high-quality assay designed for the precise measurement of interleukin-6 levels in mouse serum, plasma, and tissue culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results for a variety of research purposes.Interleukin-6 is a key cytokine involved in the regulation of immune responses and inflammation. It plays a crucial role in various physiological processes and is implicated in a range of diseases, including autoimmune disorders, cancer, and chronic inflammatory conditions.
The Mouse IL-6 CLIA Kit is an essential tool for investigating the role of IL-6 in these conditions and developing potential therapeutic strategies.With its reliable performance and ease of use, the Mouse IL-6 CLIA Kit is a valuable addition to any research laboratory focusing on immunology, inflammation, or disease pathology in mouse models. Trust Assay Genie for top-quality kits that deliver accurate and meaningful results.
Product Name:
Mouse IL-6 CLIA Kit
SKU:
MOES00041
Target:
Mouse IL-6
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-6. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IL-6 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IL-6, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse IL-6. You can calculate the concentration of Mouse IL-6 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-110
104-116
100-118
Average (%)
103
110
107
1:4
Range (%)
91-106
89-100
91-105
Average (%)
97
94
97
1:8
Range (%)
86-98
94-108
93-105
Average (%)
91
101
98
1:16
Range (%)
97-113
98-114
89-101
Average (%)
103
106
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-99
92
EDTA plasma (n=5)
92-107
98
Cell culture media (n=5)
88-101
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
25.38
43.04
236.41
25.67
46.39
259.78
Standard deviation
2.23
4.74
17.05
3.11
4.63
29.59
C V (%)
8.79
11.01
7.21
12.12
9.98
11.39
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse IL-6 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse IL-6 in samples. No significant cross-reactivity or interference between Mouse IL-6 and analogues was observed.
