The Mouse Leptin Receptor (LEPR) ELISA Kit is meticulously designed for the precise detection and quantification of leptin receptor levels in various biological samples. The leptin receptor, a key component of the leptin signaling pathway, plays a crucial role in regulating energy balance, metabolism, and appetite. This receptor is involved in mediating the effects of leptin, a hormone primarily produced by adipose tissue that regulates body weight and metabolism. Through the accurate measurement of leptin receptor levels, researchers can gain valuable insights into the mechanisms underlying metabolic homeostasis, obesity, and related metabolic disorders. The ELISA kit provides exceptional sensitivity and specificity, ensuring reliable and reproducible results that are essential for elucidating the role of the leptin receptor in metabolic regulation. Manufactured under stringent quality control standards, this ELISA kit offers robust performance and ease of use, making it an excellent choice for research focused on understanding the molecular pathways associated with energy balance and metabolic health.
Product Name:
Mouse LEPR (Leptin Receptor) ELISA Kit
SKU:
AEES00234
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
1.88 ng/mL
Detection range:
3.13-200 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
