The Mouse LZM (Lysozyme) ELISA Kit is a highly sensitive and specific assay designed for the accurate quantification of lysozyme levels in mouse serum, plasma, and cell culture supernatants. This kit offers reliable and reproducible results, making it a valuable tool for researchers studying immune responses, antimicrobial activity, and inflammatory processes in mouse models.Lysozyme is a key component of the innate immune system, exerting antimicrobial effects by breaking down bacterial cell walls. Its levels can change in response to infection, inflammation, and stress, making it a valuable biomarker for monitoring immune function and disease progression in various experimental settings.With its easy-to-use protocol and high-performance capabilities, the Mouse LZM ELISA Kit is an essential resource for investigating the role of lysozyme in immune defense mechanisms, infection responses, and inflammatory disorders in mouse models.
Product Name:
Mouse LZM (Lysozyme) ELISA Kit
SKU:
MOES01245
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
75 pg/mL
Detection range:
125-8000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
