Mouse MCP-2/CCL8 (Monocyte Chemotactic Protein 2) ELISA Kit
The Mouse MCP-2/CCL8 (Monocyte Chemotactic Protein 2) ELISA Kit is a cutting-edge assay meticulously designed for the precise and quantitative determination of MCP-2, which belongs to the monocyte chemotactic protein family, in a variety of biological samples. MCP-2, also known as CCL8, is a chemokine with potent chemoattractant properties that plays a crucial role in immune responses, specifically in the recruitment and activation of monocytes and other immune cells at sites of inflammation or injury. Accurate measurement of MCP-2 levels is essential for comprehending its involvement in the regulation of immune reactions and inflammatory processes. By quantifying MCP-2 expression, researchers can gain insights into its specific immune-modulating functions, which are fundamental in various inflammatory diseases, immune responses, and pathophysiological conditions where chemokine signaling is implicated. The Mouse MCP-2/CCL8 ELISA Kit crafted by Assay Genie delivers outstanding sensitivity and specificity, guaranteeing precise, reproducible, and dependable results. With stringent quality control measures in place, this kit ensures robust performance and ease of use, making it a top choice for research endeavors that delve into the multifaceted roles of MCP-2 in immune regulation and inflammatory pathways.
Product Name:
Mouse MCP-2/CCL8 (Monocyte Chemotactic Protein 2) ELISA Kit
SKU:
AEES00263
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
