The Mouse MCSF (Macrophage Colony-Stimulating Factor) ELISA Kit is specifically designed for the precise measurement of MCSF levels in mouse serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit delivers accurate and consistent results, making it a valuable tool for various research applications.MCSF is a key cytokine that plays a critical role in the differentiation, proliferation, and survival of macrophages. It is involved in regulating immune responses, inflammation, and tissue repair processes.
Dysregulation of MCSF levels has been linked to various diseases, including cancer, autoimmune disorders, and osteoporosis, highlighting its significance as a biomarker for scientific investigations and potential therapeutic interventions.Overall, the Mouse MCSF ELISA Kit from Assay Genie provides researchers with a reliable and efficient solution for quantifying MCSF levels, enabling comprehensive studies on macrophage biology and related pathological conditions.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse MCSF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse MCSF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse MCSF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse MCSF. You can calculate the concentration of Mouse MCSF in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-104
88-103
94-107
Average (%)
96
94
101
1:4
Range (%)
90-103
82-94
84-96
Average (%)
96
88
88
1:8
Range (%)
92-107
85-98
85-97
Average (%)
98
91
90
1:16
Range (%)
89-102
84-97
81-93
Average (%)
96
90
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-105
100
EDTA plasma (n=5)
87-99
93
Cell culture media (n=5)
95-111
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
50.7
86.24
389.29
54.41
86.56
399.03
Standard deviation
3.46
3.94
16.35
3.61
4.99
16.32
C V (%)
6.82
4.57
4.2
6.63
5.76
4.09
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse MCSF concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse MCSF in samples. No significant cross-reactivity or interference between Mouse MCSF and analogues was observed.
