The Mouse MT (Melatonin) ELISA Kit is specifically designed for the precise measurement of melatonin levels in mouse serum, plasma, and tissue culture supernatants. With its high sensitivity and specificity, this kit provides dependable and consistent results, making it a valuable tool for various research applications.Melatonin is a critical hormone involved in regulating the circadian rhythm and sleep-wake cycle. It also has antioxidant and anti-inflammatory properties, making it a key player in maintaining overall health and wellness.
Studies have shown its potential role in various physiological and pathological processes, including sleep disorders, mood disorders, and neurodegenerative diseases.By accurately measuring melatonin levels with this ELISA kit, researchers can better understand the role of melatonin in different biological processes and potentially develop new treatments or therapies targeting melatonin-related conditions.
Product Name:
Mouse MT (Melatonin) ELISA Kit
SKU:
MOES01266
Target:
Mouse MT (Melatonin)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with MT. During the reaction, MT in the sample or standard competes with a fixed amount of MT on the solid phase supporter for sites on the Biotinylated Detection Ab specific to MT. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of MT in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-110
89-100
100-113
Average (%)
101
94
106
1:4
Range (%)
87-101
91-106
97-112
Average (%)
93
98
103
1:8
Range (%)
86-100
90-101
97-109
Average (%)
92
96
104
1:16
Range (%)
88-101
93-106
94-110
Average (%)
95
99
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-102
94
EDTA plasma (n=5)
91-105
97
Cell culture media (n=5)
86-98
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
25.8
55.2
199.0
23.6
56.9
209.1
Standard deviation
1.5
2.7
10.0
1.3
3.4
9.6
C V (%)
5.81
4.89
5.03
5.51
5.98
4.59
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of MT concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes MT in samples. No significant cross-reactivity or interference between MT and analogues was observed.
