The Mouse NGF (Nerve Growth Factor) ELISA Kit is specifically designed for the precise measurement of NGF levels in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and consistent results, making it an excellent choice for various research purposes.NGF is a key protein that plays a vital role in promoting the growth and survival of nerve cells. It is essential for the development and maintenance of the nervous system, making it a crucial factor in neurobiology research. Additionally, NGF has been implicated in conditions such as Alzheimer's disease and peripheral neuropathy, underscoring its importance as a biomarker for these disorders.Overall, the Mouse NGF ELISA kit is a valuable tool for scientists studying neurotrophic factors and their role in neurodegenerative diseases, offering reliable and reproducible results for insightful research.
Product Name:
Mouse NGF (Nerve growth factor) ELISA Kit
SKU:
MOES01287
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
