The Mouse NT-proBNP (N-terminal pro-brain natriuretic peptide) ELISA Kit is specifically designed for the precise measurement of NT-proBNP levels in mouse serum, plasma, and cell culture supernatants. This kit offers unparalleled sensitivity and specificity, ensuring accurate and consistent results, making it an invaluable tool for a wide range of research purposes.NT-proBNP is a vital biomarker linked to heart failure and other cardiovascular conditions, providing valuable insights into cardiac function and prognosis. By measuring NT-proBNP levels, researchers can gain a better understanding of cardiac health and assess the effectiveness of treatments and interventions.The Mouse NT-proBNP ELISA Kit is an essential resource for studying cardiovascular diseases and developing novel therapeutic approaches, offering reliable and reproducible data to advance scientific knowledge and improve patient outcomes.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
