Mouse P1NP (Procollagen Type I N-Terminal Propeptide) ELISA Kit
The Mouse PINP (Procollagen Type I N-Terminal Propeptide) ELISA Kit is a powerful tool for the precise measurement of PINP levels in mouse serum, plasma, and tissue lysates. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.PINP is a key marker for collagen synthesis and bone formation, making it an essential component in studying bone health, osteoporosis, and other bone-related diseases.
By accurately quantifying PINP levels, researchers can gain valuable insights into the mechanisms of bone metabolism and potential interventions for these conditions.This ELISA kit simplifies the process of measuring PINP levels, providing researchers with a reliable and efficient tool for their studies. With its high performance and ease of use, the Mouse PINP ELISA Kit is an invaluable asset for any laboratory focusing on bone research and related fields.
Product Name:
Mouse P1NP (Procollagen Type I N-Terminal Propeptide) ELISA Kit
SKU:
MOES00806
Target:
Mouse P1NP (Procollagen Type I N-Terminal Propeptide)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
56.25 pg/mL
Detection range:
93.75-6000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse Pâ… NP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse Pâ… NP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse Pâ… NP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse Pâ… NP. You can calculate the concentration of Mouse Pâ… NP in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-101
92-103
88-104
Average (%)
96
97
95
1:4
Range (%)
89-102
79-89
82-95
Average (%)
97
85
88
1:8
Range (%)
90-102
82-92
85-96
Average (%)
97
87
91
1:16
Range (%)
92-103
82-95
83-99
Average (%)
97
89
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-99
91
EDTA plasma (n=5)
93-105
98
Cell culture media (n=5)
95-106
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
324.49
557.38
2793.45
353.21
570.31
2784.16
Standard deviation
20.57
23.91
126.54
24.16
32.91
126.68
C V (%)
6.34
4.29
4.53
6.84
5.77
4.55
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse Pâ… NP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse Pâ… NP in samples. No significant cross-reactivity or interference between Mouse Pâ… NP and analogues was observed.
