Rat P1NP (Procollagen Type I N-Terminal Propeptide) ELISA Kit
The Rat PINP (Procollagen Type I N-Terminal Propeptide) Elisa Kit is a reliable and accurate tool for detecting levels of PINP in rat serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, providing researchers with robust and dependable results for a variety of research applications.PINP is an important protein that plays a key role in collagen synthesis and bone formation. Monitoring PINP levels can provide valuable insights into bone metabolism, bone health, and conditions such as osteoporosis. This ELISA kit enables researchers to study the role of PINP in various physiological and pathological processes, ultimately aiding in the development of new therapeutic strategies for bone-related disorders.Overall, the Rat PINP Elisa Kit is a valuable tool for studying bone metabolism and bone health in preclinical research settings, offering researchers a reliable method for quantifying PINP levels in rat samples with high precision and accuracy.
Product Name:
Rat P1NP (Procollagen Type I N-Terminal Propeptide) ELISA Kit
SKU:
RTES00990
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
37.5 pg/mL
Detection range:
62.5-4000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.