Description
Monkeypox Virus (MPXV) A30L Protein IgA ELISA Kit
The Monkeypox Virus A30L Protein Human IgA ELISA Kit is a highly sensitive and specific assay designed for the precise detection of IgA antibodies against the A30L protein of the Monkeypox virus in human serum, plasma, and cell culture supernatants. This ELISA kit provides accurate and reproducible results, making it an essential tool for research and diagnostic purposes related to Monkeypox virus infections.The A30L protein is a key virulence factor of the Monkeypox virus, playing a critical role in the pathogenesis of the disease.
The detection of IgA antibodies against this protein can provide valuable insights into the immune response against the virus and aid in the development of effective diagnostic methods and therapeutic interventions.Overall, the Monkeypox Virus A30L Protein Human IgA ELISA Kit is a valuable resource for researchers and healthcare professionals interested in studying Monkeypox virus infections and developing strategies for prevention and treatment.
| Product Name: | Monkeypox Virus (MPXV) A30L Protein IgA ELISA Kit |
| Product Code: | MKRS0005 |
| Species Detected: | Human |
| Gene Symbols: | IGA |
| Compatible Sample Types: | Plasma, Serum |
| Design Principle: | Indirect |
| Number of Targets Detected: | 1 |
| Method of Detection: | Colorimetric |
| Quantitative/Semi-Quantitative: | Quantitative |
| Solid Support: | 96-well Microplate |
| Size: | 96T |
- Pre-coated 96-well microplate
- Wash Buffer
- Positive Control
- Biotinylated Detection Antibody
- Biotinylated Anti-Positive Control
- HRP-Streptavidin concentrate
- TMB One-Step Substrate
- Stop Solution
- Assay Diluent
- 5X Sample Diluent
Other Materials Required
- Microplate reader capable of measuring absorbance at 450 nm
- Precision pipettes to deliver 2 µl to 1 ml volumes
- Adjustable 1-25 ml pipettes for reagent preparation
- 100 ml and 1 liter graduated cylinders
- Absorbent paper
- Distilled or deionized water
- Prepare all reagents, samples and standards as instructed.
- Add 100 µl positive control or sample to each well. Incubate 1 hour at room temperature.
- Add 100 µl prepared Biotinylated detection antibody and Biotinylated Anti-Postive Control into respective wells. Incubate 1 hour at room temperature.
- Add 100 µl prepared HRP-Streptavidin solution to each well. Incubate 30 minutes at room temperature.
- Add 100 µl TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
- Add 50 µl Stop Solution to each well. Read at 450 nm immediately.
