Description
Monkeypox Virus (MPXV) M1R Protein IgM ELISA Kit
The Monkeypox Virus M1R Protein Human IgM ELISA Kit is a reliable and accurate tool for detecting IgM levels specific to the Monkeypox virus in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides precise and reproducible results, making it ideal for research and diagnostic use.The M1R protein is a key component of the Monkeypox virus, playing a crucial role in viral replication and pathogenesis. By detecting IgM antibodies against this protein, researchers can better understand the immune response to Monkeypox virus infection and develop targeted therapies and diagnostics.
This ELISA kit is essential for studying Monkeypox virus infections, monitoring outbreaks, and evaluating the effectiveness of vaccines and treatments. Its ease of use and reliable performance make it a valuable tool for researchers and healthcare professionals working in the field of virology and infectious diseases.
| Product Name: | Monkeypox Virus (MPXV) M1R Protein IgM ELISA Kit |
| Product Code: | MKRS0037 |
| Species Detected: | Human |
| Gene Symbols: | IGM |
| Compatible Sample Types: | Plasma, Serum |
| Design Principle: | Indirect |
| Number of Targets Detected: | 1 |
| Method of Detection: | Colorimetric |
| Quantitative/Semi-Quantitative: | Quantitative |
| Solid Support: | 96-well Microplate |
| Size: | 96T |
- Pre-coated 96-well microplate
- Wash Buffer
- Positive Control
- Biotinylated Detection Antibody
- Biotinylated Anti-Positive Control
- HRP-Streptavidin concentrate
- TMB One-Step Substrate
- Stop Solution
- Assay Diluent
- 5X Sample Diluent
Other Materials Required
- Microplate reader capable of measuring absorbance at 450 nm
- Precision pipettes to deliver 2 µl to 1 ml volumes
- Adjustable 1-25 ml pipettes for reagent preparation
- 100 ml and 1 liter graduated cylinders
- Absorbent paper
- Distilled or deionized water
- Prepare all reagents, samples and standards as instructed.
- Add 100 µl positive control or sample to each well. Incubate 1 hour at room temperature.
- Add 100 µl prepared Biotinylated detection antibody and Biotinylated Anti-Postive Control into respective wells. Incubate 1 hour at room temperature.
- Add 100 µl prepared HRP-Streptavidin solution to each well. Incubate 30 minutes at room temperature.
- Add 100 µl TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
- Add 50 µl Stop Solution to each well. Read at 450 nm immediately.
