The Mouse AGER (Total Advanced Glycosylation End Product Specific Receptor) ELISA Kit - High Sensitivity is a state-of-the-art assay meticulously designed for the precise quantification of the Total Advanced Glycosylation End Product Specific Receptor (AGER) levels in a variety of mouse biological samples. AGER is a vital receptor involved in mediating cellular responses to advanced glycosylation end products, playing a crucial role in inflammation, oxidative stress, and cellular signaling. By quantifying AGER levels with high sensitivity, researchers can delve into the intricate pathways where AGER exerts its regulatory functions. This high-sensitivity ELISA kit empowers researchers to unravel the complexities of AGER biology, facilitating a comprehensive understanding of its implications in diverse physiological and pathological processes. AGER's role in mediating inflammatory responses, oxidative damage, and cell signaling pathways can be thoroughly investigated, offering valuable insights into its impact on various disease conditions and potential therapeutic strategies. The Mouse AGER ELISA Kit - High Sensitivity, created by Assay Genie, ensures exceptional sensitivity and specificity, guaranteeing accurate and reproducible results for robust research outcomes. With stringent quality control measures and optimized sensitivity, this kit delivers reliable performance and user-friendly protocols, making it an indispensable tool for in-depth investigations into AGER biology and its significance in mouse models.
Product Name:
Mouse RAGE/AGER ELISA Kit (High Sensitivity)
SKU:
AEES00606
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.47 ng/mL
Detection range:
0.78-50 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
