The Mouse LEP (Leptin) ELISA Kit - High Sensitivity is a cutting-edge assay designed to quantitatively measure Leptin levels in various mouse biological samples with high precision and sensitivity. Leptin, a hormone primarily produced by adipose tissue, plays a crucial role in regulating energy balance, appetite, and metabolism, making it a key player in the body's weight and fat regulation mechanisms. With exceptional sensitivity and accuracy, this high-sensitivity ELISA kit enables researchers to elucidate the intricate interplay of Leptin in physiological processes and metabolic pathways within mouse models. Accurate quantification of Leptin is essential for deciphering its functions in energy homeostasis, obesity, and various metabolic disorders. Manufactured under stringent quality control standards, the Mouse LEP (Leptin) ELISA Kit - High Sensitivity by Assay Genie guarantees reliable and reproducible results, empowering researchers to delve deep into the intricacies of Leptin's involvement in metabolic regulation. Whether investigating obesity-related pathways or exploring Leptin's effects on metabolic health, this ELISA kit is an excellent choice for high-impact research in the field of metabolic biology.
Product Name:
Mouse Leptin ELISA Kit (High Sensitivity)
SKU:
AEES00620
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
