The Mouse MCP-1 (Monocyte Chemotactic Protein 1) ELISA Kit - High Sensitivity is a state-of-the-art assay engineered for the precise quantitative measurement of MCP-1 levels in various biological samples from mouse models. As a key cytokine involved in immune responses and inflammatory processes, MCP-1 plays a crucial role in monocyte chemotaxis, immune cell recruitment, and inflammatory cascade modulation, making it a significant target for research elucidating immune-related disorders, inflammatory conditions, and disease progression in mouse models. This high-sensitivity ELISA kit is meticulously designed to provide researchers with exceptional accuracy in assessing MCP-1 expression levels, enabling detailed investigations into its role in immune cell migration, cytokine signaling, and inflammatory pathways with unparalleled sensitivity. The enhanced sensitivity of this kit empowers researchers to explore the intricate mechanisms of MCP-1 in immune responses and inflammatory processes in mouse models with utmost precision and reliability. The Mouse MCP-1 ELISA Kit - High Sensitivity by Assay Genie ensures exceptional sensitivity and specificity, guaranteeing reliable and reproducible results. Manufactured under rigorous quality control standards, this kit delivers robust performance and ease of use, making it the preferred choice for researchers seeking to unravel the complexities of Monocyte Chemotactic Protein 1 in immune responses and inflammatory cascades in mouse models.
Product Name:
Mouse MCP-1/CCL-2 ELISA Kit (High Sensitivity)
SKU:
AEES00599
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
