The Mouse CCL12/MCP-5 (Monocyte Chemotactic Protein 5) ELISA Kit - High Sensitivity is a cutting-edge assay meticulously crafted for the precise quantitative analysis of Monocyte Chemotactic Protein 5 levels in diverse biological samples from mouse models. CCL12, a vital chemokine within the MCP subgroup, plays a pivotal role in immune cell recruitment, inflammation modulation, and various immunological responses, making it a key target for investigations in immune-related conditions, angiogenesis regulation, and tissue repair mechanisms. This high-sensitivity ELISA kit is engineered to empower researchers with unparalleled accuracy in determining CCL12/MCP-5 expression, shedding light on its intricate involvement in immune cell migration, cytokine signaling, and inflammatory pathways with exceptional sensitivity. The enhanced sensitivity of this kit enables researchers to explore the multifaceted roles of CCL12 in immune responses and inflammatory cascades in mouse models with superior precision. The Mouse CCL12/MCP-5 ELISA Kit - High Sensitivity by Assay Genie delivers outstanding sensitivity and specificity, ensuring dependable and reproducible outcomes. Crafted under stringent quality assurance protocols, this kit guarantees robust performance and user-friendly operation, making it the ideal solution for research endeavors aiming to decipher the complexities of Monocyte Chemotactic Protein 5 in immune modulation and inflammatory processes in mouse models.
Product Name:
Mouse CCL12/MCP-5 ELISA Kit (High Sensitivity)
SKU:
AEES00598
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
3.97 pg/mL
Detection range:
7.81-500 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
