Mouse CCL12/MCP-5 (Monocyte Chemotactic Protein 5) ELISA Kit
The Mouse CCL12/MCP-5 (Monocyte Chemotactic Protein 5) ELISA Kit is a specialized assay designed to quantitatively measure the levels of MCP-5 in various biological samples sourced from mouse models. MCP-5, also known as CCL12, is a chemokine known for its role in immune responses and monocyte chemotaxis, making it a key mediator in inflammatory processes and immune cell recruitment. This ELISA kit by Assay Genie is tailored to offer exceptional sensitivity and specificity, ensuring accurate and reproducible quantification of MCP-5 levels. With stringent quality control measures in place and validated protocols, researchers can rely on this kit for robust performance and precise results. The user-friendly design of the kit further enhances its usability, making it a valuable tool for research aimed at understanding the functions and implications of MCP-5 in inflammatory responses and related biological processes. Trust in Assay Genie's Mouse CCL12/MCP-5 ELISA Kit for reliable and insightful data in your research endeavors.
Product Name:
Mouse CCL12/MCP-5 (Monocyte Chemotactic Protein 5) ELISA Kit
SKU:
AEES00200
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
37.5 pg/mL
Detection range:
62.5-4000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
