The Mouse MMP-8 (Matrix Metalloproteinase 8) ELISA Kit - High Sensitivity is a cutting-edge research tool meticulously crafted for the precise quantitative analysis of Matrix Metalloproteinase 8 (MMP-8) levels in diverse biological samples from mouse models. MMP-8, a pivotal member of the matrix metalloproteinase family, plays a crucial role in extracellular matrix remodeling, tissue repair, and inflammation modulation. By quantifying MMP-8 levels with high sensitivity, researchers can gain profound insights into its involvement in tissue homeostasis, pathological conditions, and immune responses in mice. This high-performance ELISA kit provides researchers with a powerful means to explore the intricate functions of MMP-8 in biological processes, offering a detailed understanding of its impact on tissue remodeling, immune modulation, and disease progression in mouse models. The precise measurement of MMP-8 levels facilitates the comprehensive investigation of its role in various physiological and pathological contexts, shedding light on its potential therapeutic implications in diverse conditions. The Mouse MMP-8 ELISA Kit - High Sensitivity, developed by Assay Genie, delivers exceptional accuracy and reproducibility, ensuring the generation of reliable data for insightful research outcomes. Manufactured under rigorous quality control standards and optimized for high sensitivity, this kit guarantees robust performance and user-friendly protocols, making it an invaluable asset for researchers delving into MMP-8 biology and its implications in mouse models.
Product Name:
Mouse MMP-8 ELISA Kit (High Sensitivity)
SKU:
AEES00605
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.09 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.