The Mouse TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit - High Sensitivity is a cutting-edge assay designed for the precise and quantitative measurement of Tumor Necrosis Factor Alpha (TNF-α) levels in various mouse biological samples. TNF-α is a critical pro-inflammatory cytokine that plays a pivotal role in various cellular processes such as inflammation, cell proliferation, immune response modulation, and cell death. This high-sensitivity ELISA kit offers unparalleled accuracy and reliability in TNF-α detection, enabling researchers to delve into the intricate roles of TNF-α in disease mechanisms, autoimmune disorders, cancer, and infections. With exceptional sensitivity and specificity, this kit provides reproducible results, empowering scientists to explore the complexities of TNF-α biology and develop targeted therapeutic strategies. Manufactured under stringent quality control standards, the Mouse TNF-α ELISA Kit - High Sensitivity delivers robust performance and user-friendly protocols, making it an indispensable tool for cutting-edge research in immunology, inflammation, and cytokine biology utilizing mouse models.
Product Name:
Mouse TNF-alpha ELISA Kit (High Sensitivity)
SKU:
AEES00589
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
