The Mouse TNFRSF1B (Tumor Necrosis Factor Receptor Superfamily, Member 1B) ELISA Kit - High Sensitivity is a cutting-edge assay engineered for the precise quantitative analysis of TNFRSF1B levels in diverse biological samples extracted from mouse models. TNFRSF1B, a vital member of the tumor necrosis factor receptor superfamily, serves as a key receptor involved in signaling pathways associated with inflammatory responses and immune regulation. This high-sensitivity ELISA kit enables accurate quantification of TNFRSF1B, shedding light on its pivotal role in orchestrating cellular processes linked to inflammation, cell survival, and immune modulation within murine systems. Accurate measurement of TNFRSF1B levels is integral for unraveling its impact on immune responses, inflammation-related pathways, and disease pathogenesis. The Mouse TNFRSF1B ELISA Kit - High Sensitivity by Assay Genie guarantees exceptional sensitivity and specificity, ensuring trustworthy and reproducible results. Manufactured under stringent quality control standards, this ELISA kit delivers robust performance and user-friendly operation, making it an optimal choice for researchers investigating the functions and implications of TNFRSF1B in diverse biological contexts.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
