The pan-Symmetric-Di-Methyl Arginine Antibody (CAB18261) is a high-quality antibody developed for reliable detection and analysis of target proteins. The methylation of arginine residues in proteins is a post-translational modification that contributes to a wide range of biological processes. Many cytokines involved in T cell development and activation utilize the common cytokine receptor γ-chain (γc) and the kinase JAK3 for signal transduction.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat, Other (Wide Range Predicted) samples.
Product Name:
pan-Symmetric-Di-Methyl Arginine Antibody
SKU:
CAB18261
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat, Other (Wide Range Predicted)
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:100 - 1:500
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Sample:
HeLa, MCF7, NIH/3T3, C6, Mouse liver, Rat liver
Observed MW:
22-70kDa
The methylation of arginine residues in proteins is a post-translational modification that contributes to a wide range of biological processes. Many cytokines involved in T cell development and activation utilize the common cytokine receptor γ-chain (γc) and the kinase JAK3 for signal transduction.
Purification Method
Affinity purification
RRID
AB_2862036
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using pan-Symmetric-Di-Methyl Arginine Rabbit pAb (CAB18261) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Dot-blot analysis of all sorts of methylation peptides using pan Symmetric-DiMethyl-Arginine antibody (CAB18261) at 1:1000 dilution.
