Anti-Phospho-AKT1-S473+AKT2-S474+AKT3-S472 Antibody (CABP1068)

SKU:
CABP1068
€99 - €319

Description

Technical Manual

Anti-Phospho-AKT1-S473+AKT2-S474+AKT3-S472 Antibody

Product Name:Anti-Phospho-AKT1-S473+AKT2-S474+AKT3-S472 Antibody
Product SKU:CABP1068
Size:20uL, 50 uL
Host Species:Rabbit
Purification Method:Affinity purification
Isotype:IgG
Background:

The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2011]

Immunogen:A phospho synthetic peptide corresponding to residues surrounding S473/S474/S472 of human AKT1/AKT2/AKT3.
Sequence:

Email for sequence

Gene ID:207/208/10000
Swiss Prot:P31749/P31751/Q9Y243
Synonyms:AKT1/AKT2/AKT3
Calculated MW:48kDa/55kDa/51kDa/54kDa
Observed MW:60kDa
Reactivity:Human, Mouse, Rat
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting Immunohistochemistry 
Positive Samples:NIH/3T3, C6
Cellular Location:

Product Images

Anti-Phospho-AKT1-S473+AKT2-S474+AKT3-S472 Antibody (CABP1068)

Western blot - Phospho-AKT1-S473+AKT2-S474+AKT3-S472 Rabbit pAb (CABP1068)
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Additional Information

Product type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
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