The CDC37 Antibody (CAB2859) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By targeting the CDC37 protein, this antibody allows for precise detection and analysis in various cell types, making it an ideal tool for studies in cell biology and cancer research.CDC37 is a crucial regulator of protein kinase activity, playing a key role in the folding and maturation of these important signaling molecules.
This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
CDC37 Antibody
SKU:
CAB2859
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
P50CDC37, CDC37
Positive Sample:
Raji, HeLa, HepG2, BT474, HT-1080
Cellular Localization:
Cytoplasm.
Calculated MW:
44kDa
Observed MW:
50kDa
The protein encoded by this gene is highly similar to Cdc 37, a cell division cycle control protein of Sacchromyces cerevisiae. This protein is a molecular chaperone with specific function in cell signal transduction. It has been shown to form complex with Hsp90 and a variety of protein kinases including CDK4, CDK6, SRC, RAF-1, MOK, as well as eIF2 alpha kinases. It is thought to play a critical role in directing Hsp90 to its target kinases.
Purification Method
Affinity purification
Gene ID
11140
RRID
AB_2764684
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using CDC37 Rabbit pAb (CAB2859) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
