Anti-Phospho-PXN-Y118 Antibody (CABP1057)

SKU:
CABP1057
€99 - €319

Description

Technical Manual

Anti-Phospho-PXN-Y118 Antibody

Product Name:Anti-Phospho-PXN-Y118 Antibody
Product SKU:CABP1057
Size:20uL, 50 uL
Host Species:Rabbit
Purification Method:Affinity purification
Isotype:IgG
Background:

This gene encodes a cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). Alternatively spliced transcript variants encoding different isoforms have been described for this gene. These isoforms exhibit different expression pattern, and have different biochemical, as well as physiological properties (PMID:9054445).

Immunogen:A phospho synthetic peptide corresponding to residues surrounding Y118 of human PXN.
Sequence:

Email for sequence

Gene ID:5829
Swiss Prot:P49023
Synonyms:Paxillin; PXN; paxillin
Calculated MW:64kDa
Observed MW:64kDa
Reactivity:Human, Mouse
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting
Positive Samples:HeLa
Cellular Location:Cell junction, Cytoplasm, cell cortex, cytoskeleton, focal adhesion

Product Images

Anti-Phospho-PXN-Y118 Antibody (CABP1057)

Western blot - Phospho-PXN-Y118 Rabbit pAb (CABP1057)

Uniprot Information

UniProt Protein Function:PXN: a multi-domain cytoskeletal protein involved in actin-membrane attachment that localizes primarily to focal adhesion sites to the extracellular matrix. Phosphorylated by focal adhesion kinase (FAK) and is a component of integrin signaling. Its phosphorylation provides docking sites for recruitment of signaling molecules to focal adhesions. Binds in vitro to vinculin as well as to the SH3 domain of c-SRC and, when tyrosine phosphorylated, to the Crk SH2 domain. Interacts with GIT1, NUDT16L1/SDOS, PARVA and TGFB1I1. Component of cytoplasmic complexes, which also contain GIT1, ARHGEF6 and PAK1. Binds DDEF2. Interacts with unphosphorylated ITGA4. Interacts with RNF5.Three alternatively-spliced isoforms have been described. Isoform beta binds to focal adhesion kinase but weakly to vinculin. Isoform gamma binds to vinculin but only weakly to focal adhesion kinase.
UniProt Protein Details:

Protein type:Adaptor/scaffold; Cytoskeletal; Motility/polarity/chemotaxis; Cell adhesion

Chromosomal Location of Human Ortholog: 12q24.31

Cellular Component: nucleoplasm; microtubule associated complex; focal adhesion; lamellipodium; cytoplasm; stress fiber; plasma membrane; cell cortex; cytosol

Molecular Function:integrin binding; protein binding; zinc ion binding; beta-catenin binding; BH4 domain binding; protein kinase binding; vinculin binding

Biological Process: integrin-mediated signaling pathway; lamellipodium biogenesis; epidermal growth factor receptor signaling pathway; focal adhesion formation; regulation of cell shape; peptidyl-tyrosine phosphorylation; branching morphogenesis of a tube; muscle contraction; activation of MAPK activity; transforming growth factor beta receptor signaling pathway; signal complex assembly; cytoskeleton organization and biogenesis; vascular endothelial growth factor receptor signaling pathway; signal transduction; cell adhesion

NCBI Summary:This gene encodes a cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). Alternatively spliced transcript variants encoding different isoforms have been described for this gene. These isoforms exhibit different expression pattern, and have different biochemical, as well as physiological properties (PMID:9054445). [provided by RefSeq, Aug 2011]
UniProt Code:P49023
NCBI GenInfo Identifier:317373486
NCBI Gene ID:5829
NCBI Accession:P49023.3
UniProt Secondary Accession:P49023,O14970, O14971, O60360, Q5HYA4, B2RAI3, B7ZMB4
UniProt Related Accession:P49023
Molecular Weight:68kDa
NCBI Full Name:Paxillin
NCBI Synonym Full Names:paxillin
NCBI Official Symbol:PXN
NCBI Protein Information:paxillin
UniProt Protein Name:Paxillin
Protein Family:Paxillin
UniProt Gene Name:PXN
UniProt Entry Name:PAXI_HUMAN
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Additional Information

Product type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
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