The Rat AChE (Acetylcholinesterase) ELISA Kit is specifically designed for the quantitative analysis of acetylcholinesterase levels in various rat biological samples. Acetylcholinesterase is a vital enzyme involved in the hydrolysis of the neurotransmitter acetylcholine, thereby contributing to the termination of synaptic transmission at cholinergic synapses. The precise regulation of acetylcholinesterase activity is crucial for maintaining proper neuronal signaling, neuromuscular function, and cognitive processes. In various tissues, particularly in the nervous system, acetylcholinesterase activity is tightly controlled to ensure accurate neurotransmission and prevent the accumulation of acetylcholine. Dysregulation of AChE levels or activity can lead to neurological disorders, cognitive impairments, and neuromuscular dysfunction. The Rat AChE ELISA Kit from Assay Genie provides researchers with a reliable and accurate tool to measure acetylcholinesterase levels with exceptional sensitivity and specificity. This kit is manufactured under rigorous quality control standards to ensure dependable performance and reproducible results, making it an excellent choice for studies focusing on cholinergic neurotransmission, neurodegenerative diseases, and neurobiology research.
Product Name:
Rat AChE (Acetylcholinesterase) ELISA Kit
SKU:
AEES00387
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.47 ng/mL
Detection range:
0.78-50 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
