The Rat Alpha-1-AT (Alpha-1 Antitrypsin) ELISA Kit is a reliable tool for accurately measuring levels of Alpha-1 Antitrypsin in rat serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.Alpha-1 Antitrypsin is a critical protein that plays a key role in protecting tissues from damage caused by enzymes released during inflammation. Dysregulation of Alpha-1 Antitrypsin has been linked to various diseases, including liver and lung disorders. Therefore, monitoring Alpha-1 Antitrypsin levels can provide valuable insights into disease progression and potential therapeutic interventions.Overall, the Rat Alpha-1-AT ELISA Kit is an indispensable tool for researchers studying the role of Alpha-1 Antitrypsin in health and disease, offering a reliable and efficient method for quantifying its levels in biological samples.
Product Name:
Rat alpha1-AT (Alpha 1-Antitrypsin) ELISA Kit
SKU:
RTES01048
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
3.75 ng/mL
Detection range:
6.25-400 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
