The Human Alpha-1-AT (Alpha-1 Antitrypsin) ELISA Kit is specifically designed for the accurate measurement of alpha-1 antitrypsin levels in human serum, plasma, and cell culture supernatants. This kit is highly sensitive and specific, ensuring precise and consistent results for a variety of research applications.Alpha-1 antitrypsin is a critical protein that plays a key role in protecting tissues from enzymes that can cause damage. Deficiencies in alpha-1 antitrypsin have been linked to conditions such as chronic obstructive pulmonary disease (COPD), liver cirrhosis, and emphysema. Therefore, this ELISA kit is essential for studying these conditions and developing potential treatments.Overall, the Human Alpha-1-AT (Alpha-1 Antitrypsin) ELISA Kit offers researchers a reliable tool for accurately measuring alpha-1 antitrypsin levels, enabling a better understanding of its role in various diseases and potential therapeutic interventions.
Product Name:
Human alpha1-AT (Alpha 1-Antitrypsin) ELISA Kit
SKU:
HUES02087
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
1.88 ng/mL
Detection range:
3.13-200 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
